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Literature summary for 1.3.1.84 extracted from

  • Loder, A.J.; Han, Y.; Hawkins, A.B.; Lian, H.; Lipscomb, G.L.; Schut, G.J.; Keller, M.W.; Adams, M.W.; Kelly, R.M.
    Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea (2016), Metab. Eng., 38, 446-463 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.84

Activating Compound

Activating Compound Comment Organism Structure
ATP required Metallosphaera sedula

Cloned(Commentary)

Cloned (Comment) Organism
gene Msed_1426, cloned from genomic DNA, recombinant co-expression of His-tagged enzyme with enzyme succinic semialdehyde reductase (NADPH), SSR, gene Msed_1424 from Metallosphaera sedula, in Escherichia coli strain Rosetta 2(DE3). Construction of a yeast two-hybrid assay for protein interaction analysis of ACR and SSR. ACR cannot be expressed in soluble, active form in Escherichia coli Metallosphaera sedula

Protein Variants

Protein Variants Comment Organism
additional information metabolic engineering analysis of the the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle for improving the pathway flux, overview Metallosphaera sedula

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Metallosphaera sedula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acrylyl-CoA + NADPH + H+ Metallosphaera sedula
-
 propanoyl-CoA + NADP+
-
 r
acrylyl-CoA + NADPH + H+ Metallosphaera sedula DSM 5348
-
 propanoyl-CoA + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula A4YGN2
-
-
Metallosphaera sedula DSM 5348 A4YGN2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2(DE3) by immobilized metal affinity chromatography and gel filtration Metallosphaera sedula

Reaction

Reaction Comment Organism Reaction ID
propanoyl-CoA + NADP+ = acryloyl-CoA + NADPH + H+ the enzyme followes an ordered Bi-Bi reaction mechanism Metallosphaera sedula
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylyl-CoA + NADPH + H+
-
 Metallosphaera sedula propanoyl-CoA + NADP+
-
 r
acrylyl-CoA + NADPH + H+
-
 Metallosphaera sedula DSM 5348 propanoyl-CoA + NADP+
-
 r
additional information the enzyme activity is assayed coupled to 3-hydroxypropionyl-CoA synthetase (AMP-forming) Metallosphaera sedula ?
-
 ?
additional information the enzyme activity is assayed coupled to 3-hydroxypropionyl-CoA synthetase (AMP-forming) Metallosphaera sedula DSM 5348 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, recombinant His-tagged enzyme, SDS-PAGE Metallosphaera sedula
More quaternary structure analysis of ACR Metallosphaera sedula

Synonyms

Synonyms Comment Organism
Acr
-
 Metallosphaera sedula
acryloyl-CoA reductase
-
 Metallosphaera sedula
acryloyl-CoA reductase (NADPH)
-
 Metallosphaera sedula
Msed_1426
-
 Metallosphaera sedula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 assay at Metallosphaera sedula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Metallosphaera sedula

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Metallosphaera sedula

General Information

General Information Comment Organism
metabolism the enzyme is part of the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle fixes CO2 in extremely thermoacidophilic archaea, pathway regulation and modeling, enzyme kinetics-based models, reaction kinetics model of the 3HP/4HB cycle, detailed overview Metallosphaera sedula
additional information two-hybrid assay analysis of the protein interaction of ACR and SSR in Saccharomyces cerevisiae Metallosphaera sedula